ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2015, Vol. 46 ›› Issue (2): 303-308.doi: 10.11843/j.issn.0366-6964.2015.02.018

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The Distribution of PGP9.5 and NPY in the Normal Testis and Cryptorchidism of Bactrian Camel

YUAN Li-gang1*,YAN Zhen-long2,TAO Jin-zhong3,LIU Ying1*,CHEN Guo-juan1   

  1. (1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou 730070,China;2.College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China;3.College of Veterinary Medicine,Ningxia University,Yinchuan 750021,China)
  • Received:2014-06-16 Online:2015-02-23 Published:2015-02-23

Abstract:

The aim of the present study was to investigate the expression of PGP9.5 and NPY in the normal testis and cryptorchidism of Bactrian camel.The cryptorchidism and the normal testis of bactrian camel were studied by immunohistochemical SP technique and IPP (Image-Pro Plus) statistics method.The results showed that the PGP9.5 immunoreactive products distributed strongly in the Sertoli cells,sperm cells,arteries and veins of the normal testis,as well as NPY was moderate positive in Sertoli cell,sperm cells and arteries but weak positive in veins,in addition,Leydig cells in adult camels were strongly immunostained with NPY not only in normal testis but also in cryptorchidism.However,immunoreactivity of PGP9.5 and NPY were detected in cryptorchidism testis with significant differences intensities compared with the normal.These findings suggest that PGP9.5 and NPY are detected in the majority of cells in the seminiferous tubules and the blood vessels are involved in spermatogenesis and secretion of testosterone in Bactrian camel testis,they may play important roles in Bactrian camel male fertility and infertility.

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